Activity of Various Fractions of Saussurea Lappa Herb Against Multidrug Resistant Gram Negative Rods : S. Lappa against MDR Gram Negative Rods

Iqra Tabassum; Sidrah Saleem; Irfan Ahmad; Usman Arshad

doi:10.37978/tijfs.v6i1.407

Authors

Iqra Tabassum Department of Microbiology, University of Health Sciences, Lahore, Pakistan Author https://orcid.org/0000-0001-7116-4246
Sidrah Saleem Department of Microbiology, University of Health Sciences, Lahore, Pakistan Author
Irfan Ahmad Department of Biomedical and Allied Health Sciences, University of Health Sciences, Lahore, Pakistan, Department of Molecular Biology, Faculty of Medicine, Umea University, Umea, Sweden Author
Usman Arshad Department of Microbiology, University of Health Sciences, Lahore, Pakistan Author

DOI:

https://doi.org/10.37978/tijfs.v6i1.407

Keywords:

Sassura Lappa Herb, MDR, Multidrug Resistance, Gram Negative Rods, GNR, Carbapenemase producing organisms, Methanol, n-hexane, chloroform

Abstract

Background: Microorganisms are turning out to be greatly resistant to existing antibiotics, specifically gram-negative rods which shows resistance to currently accessible antibiotics. Beta-lactam antibiotics are the main therapeutic option to treat infections of gram-negative microorganisms i.e., ESBL and Carbapenemase producers. Saussurea lappa herb is a medicinal herb use since many times. Basically, the roots of S. lappa herb were used as medicines. Current study was conducted to find out activity of various fractions of S. lappa herb against multidrug resistant gram-negative rods.

Methods: Crude extracts of ethanol, methanol and water and fractions of n-hexane, chloroform, and methanol from S. lappa herb were used. Total of sixty multidrug resistant organisms were included in which thirty were ESBL and thirty were carbapenemase producing organisms. Mean MIC and comparative analysis of various extracts and fractions have been evaluated.

Results: The mean MIC value of crude extracts of ethanol, methanol, n-hexane, chloroform and methanol fractions and water extract from S. lappa against ESBL producing organisms were 109.33± 6.915 mg/ml, 154.67± 5.164 mg/ml, 150.00± 5.345 mg/ml, 55.33±5.164 mg/ml, 178.00±6.103 mg/ml, 64.00± 7.701 mg/ml respectively. Similarly, the mean MIC value against carbapenemase producing organisms were 100.67± 8.683 mg/ml, 158.67± 3.519 mg/ml, 150.67± 5.936 mg/ml, 54.67±5.164 mg/ml, 176.67±14.223 mg/ml, 64.33± 9.353 mg/ml respectively.

Conclusion: This study suggests that extracts and fractions of S. lappa herb can form the basis to develop novel broad-spectrum formulation for antimicrobial drugs as it contains compounds that has novelty to perform its action against multi-resistant mechanisms.

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